Functionalization of additive-manufactured Ti6Al4V scaffolds with poly(allylamine hydrochloride)/poly(styrene sulfonate) bilayer microcapsule system containing dexamethasone

Porous titanium alloy Ti6Al4V scaffolds manufactured via electron beam melting (EBM®) reveal broad prospects for applications in bone tissue engineering. However, local inflammation and even implant failure may occur while placing an into the body. Thus, application of drug carriers to surface a metallic can provide treatment at site. In this study, we propose use polyelectrolyte (PE) microcapsules formed by layer-by-layer (LbL) synthesis loaded with both porous calcium carbonate (CaCO3) microparticles anti-inflammatory dexamethasone (DEX) functionalize surfaces achieve controlled release. Scanning microscopy indicated that CaCO3 coated PE bilayers DEX had spherical shape diameter 2.3 ± 0.2 μm entire scaffold was evenly microcapsules. UV spectroscopy showed LbL allows manufacturing 40% DEX. According high performance liquid chromatography (HPLC) analysis, 80% released within 24 h from capsules consisting three polystyrene sulfonate (PSS) poly(allylamine)hydrochloride (PAH). The prepared functionalized hydrophilic properties water contact angle below 5°. Mouse embryonic fibroblast cells were seeded on without modification. modification core affected cell morphology vitro. results confirmed no toxic effect did not prevent adhesion spreading, thus cytotoxic observed, which will be further studied vivo.